Measurement of Prothrombin Time and Activated Partial Thromboplstin Time among Johnson and Johnson COVID-19 Vaccine Recipients in The Military Hospital in Khartoum State

Abstract

Johnson and Johnson COVID-19 vaccine although mostly safe and effective, has the potential risk of developing blood clots, alongside other possible side effects. This was analytical cross-sectional study to analyze the effect of Johnson and Johnson COVID-19 vaccine on prothrombin time (PT) and activated partial thromboplastin time (APTT) among the vaccine recipients. The study was conducted in the vaccination center in the military hospital in Khartoum State, during the period between March 2022 to June 2022. The study population compromise of 52 recipients of Johnson and Johnson COVID-19 vaccine, samples were taken before and after the administration of the vaccine. The participants’ age varies from 19 to 61 years old with 38 males and 14 females.Data was collected using structured questionnaire which included general information about the participants and a venous blood sample 1.8ml was collected in 3.2% tri sodium citrate container from each participant. Then, plasma was separated by centrifugations for 15 minutes and estimation of the PT and APTT was performed by using semi –automated biochemical analyzer (ES-105). The collected data was analyzed by using SPSS ver 26. The results showed that the pre vaccine mean level of PT and APTT was 13.8 ± SD1.2 and 33.54 ± SD 3.4 respectively. Whereas the post vaccine means level of PT and APTT was 13.9 ± SD 1.4 and 33.46 ± SD 2.75 respectively. Showing that the difference between pre and post mean levels of the two tests were very small and were statistically not significant. The respective p-values were 0.361 and 0.810. The results showed that age, gender, posttest date and the interaction between gender and posttest date had no significant effects on the differences between pre and post levels of each of the two tests (PT& APTT). The study concluded that the mean level of PT and APTT before and after the administration of Johnson and Johnson COVID-19 vaccine were statistically not significant and that age, gender, posttest date and their interaction had no significant effects on the before and after levels of each of the two tests (PT& APTT).