Antimicrobial Susceptibility and Molecular Detection of Carbapenem Resistant Genes NDM-1 and IMP-1 of Pseudomonas aeruginosa Isolated from Clinical Specimens in Khartoum State-Sudan

Abstract

Carbapenems are the most powerful β-lactam antibiotics against bacteria especially multidrug resistant isolates like Pseudomonas aeruginosa (P.aeruginosa). This study was conducted to detect carbapenem resistance genes of P. aeruginosa from clinical specimens in Khartoum State in period from January _ June). A total of Fivety (n=50) isolates of p. aeruginosa were included in this descriptive cross sectional laboratory based study. P aeruginosa were isolated from urine, blood, wound swab , sputum and Body fluids samples, which were obtained from different hospitals in Khartoum State. The samples were collected from both males and females with different age using non-self-constructing information list. The isolated organism was stored in 20% Glycerol peptone media and was inoculated on CLED agar. Antibiotic susceptibility tests were carried out using Kirby Bauer technique. DNA was extracted using boiling method. The isolates were tested for the presence of carbapenem resistance genes (NDM, IMP) using Multiplex PCR. Out of 50 P. aeruginosa isolates, 19 (38%) were positive for one or both carbapenem resistance genes; 6 (12%) were positive for NDM gene and 13(26%) were positive for IMP gene. Tow isolates possess both genes. The study concluded that, there was a high percentage and multiplicity of carbapenem resistance genes among P. aeruginosa isolates in Khartoum State.