Abstract:
This study aimed to determine the optimum conditions for production of ethanol using sugar cane under enzymatic catalysis of favorable kinetic parameters; duration, temperature, pH, enzyme concentration have been studied.
Fibers were manually removed ready sugar cane juice. The juice obtained was sterilized by heating at temperature of 90ºC for 40 minutes, and then cooled. Octapol powder (3g), was added to (1 L) of juice. Then the sample was filtered. Polarimeter device was used to determine sugar percentage. Three grams of sodium metabisulphite (Na2S2O4) were added to prevent the growth of undesirable microorganisms. The yeast was added to the substrate and was properly mixed and shaked, then left for 10 minutes. The results showed that maximum rate of alcohol produced was (2.42) after 90 minutes of fermentation, the optimal temperature was 34 C°, the optimal substrate concentration was (85.7%) at pH 5, and one gram of yeast.
