Assessment of the Effect Plasma Storage Conditions on Prothrombin Time

Abstract

Prothrombin time is the most frequently ordered test in clinical coagulation laboratory, it is used to assess the integrity of tissue factors pathway of coagulation, more over it is test of choice (reported INR) for monitoring patients on long term anticoagulant therapy. Furthermore it is most sensitive test for assessment of severity of liver disease. This is cross sectional study carried out in Khartoum state in Advanced Diagnostic laboratory, in the period from April to December 2019thirty samples collected from normal health individuals. Plasma sample were collected from volunteers for estimation of, PT using semi- automated analyzer (stago),data was analysed by SPSS version 23. The sample measure at (0,6,12,24) in RT & REF the PT measured at 6 hrRT, 12 REF and 24 freezingshowed in significant difference when compared with measurement at 0hrs while the difference were significant with measurement at 6hrs REF and 12hrs at RT. INR result show no significant up to 6 hrs at RT/REF and 24 hrsfreezing.Butsignificantafter12hrsinRTandREF.