A Study Of Molecular Detection of Helicobacter pylori IceA gene in Gastric biopsy from Endoscopic patients in AL Faisal and AL Ribat Hospitals in Khartoum State

Abstract

Colonization with Helicobacter pylori known to be cause of variety of clinical consequences includes gastritis or even gives rise to severe and potentially fatal complications, and different studies suggested that there is a relationship between the clinical outcomes and some virulence gene that H.pyloi has. The aim of this study is to detect 16s rRNA gene and the virulence gene iceA of H.pylori among Sudanese patients in Khartoum State. The samples were collected from 2 hospitals in Khartoum, AL Faisal and AL Ribat Hospitals. The participants were aged from 15 to 73 years old who visited our target hospitals for endoscopy procedure, which revealed 88.3% with gastritis and 11.3% with gastric ulcer, and the majority of patients in the study (68.8%) claimed to have a previous H. pylori infection. Total of eighty (N=80) gastric biopsy specimens were collected from patients, the gender distribution was (50%) males and (50%) females, the males had the most of the positive results for the 16s rRNA ( )and iceA genes ( ), among the samples eleven (13.8%) were confirmed as H.pylori positive by PCR by the presence of 16s rRNA of H.pylori, and from the positive samples seven (8.8%) were positive also by PCR for iceA gene. In the current study it was found there was no statistically significant association between H.pylori and its iceA virulence gene with age or gender. Regarding clinical outcome; no significant association (p value= .129) has been detected between iceA gene and gastric ulcer, while there was a significant association (p value= .005) between 16s rRNA gene of H.pylori and gastric ulcer. The study confirmed the relationship between H. pylori and gastric ulcer. Further studies are required to consolidate these results with larger samples.