Effect of Storage Period on CBC in Whole Blood Bags in Blood Bank that Containing CPDA-1 as Anticoagulant in East Nile locality

Abstract

This is analytical study conducted in Umdownban Hospital East Nile locality -Khartoum State during March to December 2019. To evaluate the effect of storage period on complete blood count in whole blood bag in blood bank which containing citrate phosphate dextrose -1 as anticoagulant. Forty healthy male blood donors, aged 27± years, who attended Umdownban Hospital during the study period, were enrolled in the study. Participation was voluntary and an informed verbal consent had been taken from each participant before commencement of the study. Physiological tests, ABO blood group and viral screening have been done as routine tests for donation, collected blood bags were placed at 4-6°C for one month, anticoagulated whole blood was taken from each bag at day one, after 15 days and 30 days of collection. Blood was analyzed by Mindray BC-3000 Plus autohematological analyzer. The result were analyzed by SPSS version 20. ANOVA test was performed. The mean values of RBCs Count, Hb and HCT were not affected by storage. MCV was significantly (P=0.000) increased with the length of the storage period and the highest value (97.2±9.55fL) was found at day thirty. A significant decreased in MCHC was observed among the three periods the lowest value was (27.2±3.11g/dl) found in day thirty. The two storage periods registered significantly higher values (P=0.000) for RDW.CV than day one, and no significant variation was found between the two periods. RDW.SD% (P=0.014) value of day 30 was significantly higher than that of day one. Day 15 and 30 registered significantly lower values for WBCs than day one, and no significant variation was found between them. The lymphocytes count was significantly (P=0.000) increased with the length of the storage period and the highest value (73.6±17.65%) was found at day thirty. The granulocytes count was significantly (P=0.000) decreased with storage and the lowest value (26.5±14.78%) was found at day fifteen. Platelet count was significantly decreased with the length of the storage periods and the lowest value was (121±54.2 × 109cell/ L) registered in day thirty. PDW value of day thirty was significantly higher than that of day one. PCT value of day 30 was significantly lower than that of day one. No significant variation was found between day 15 and 30 with regard to PDW or PCT. MPV was not affected significantly (P≤ 0.05) with the storage. It is concluded that storage significantly effects some parameters of the CBC and the effect increases with the length of the storage periods