Molecular Detection of Helicobacter pylori and Human Papilloma Virus Genotype 18 in Gastric Biopsies of Patients with Gastric Problems Attending Police Hospital - Khartoum

Abstract

A growing amount of evidence has shown that the Human Papilloma virus (HPV) infection and H.pylori directly or indirectly, can result in numerous malignant tumors. This is a descriptive cross-sectional study conducted at the period from January 2019 to February 2020 which aimed to detect HPV-18 infection and H.pylori in gastric biopsy among Sudanese patients. Gastric biopsies were collected from 100 patients suffering from gastric problems with age ranged from 15 to 80 years with mean age 42.21± 16.8 S.D. Included in this study in which 61 (61%) of them were males and 39 (39%) were females. The gastric disorders in this study included gastritis (57%), gastric ulcer (19%), duodenitis (14%), duodenal ulcer (1%), gastric erosion (6%), and abnormal tissue (3%). DNA Extraction was done manual and the H.pylori and HPV-18 DNA were amplified for each sample using PCR and agarose gel electrophoresis was used to detect the PCR products. PCR results yielded 34/100 (34%) positive in case of H.pylori and negative result with HPV-18 in all gastric biopsy samples. Concerning gender, 20/61 (58.8%) males and 14/39 (41%) females were positive for H. pylori, and there was no significant association between H.pylori infection and gender (p = 0.749). Regarding age groups ,the highest frequency was 12/100 (12%) among patients with age group 15- 30 years ,followed by 40-60 years (9%), 31-45 years (8%), 61-75 years (4%) and the lowest frequency at age ≥76 years (1%).There was no statistically significant difference between H. pylori infection and age groups (P=0.729) . This study concluded the H.pylori was more frequent among patients with gastritis; there was no detected HPV-18 among patients with gastric problems while H.pylori was detected in rated frequency (34%), there was no significant association between age, gender, and H.pylori infection. There was a significant association between gastric problems and H.pylori infection (P= 0.017).