Antimicrobial Activity of Vitis vinifera Leaves Extracts against Multidrug Resistance Bacteria Isolated from Sudanese Patients with Diabetic Foot Infection

Abstract

An alarming increase in bacterial strains resistant to existing antibacterial agents demands a renewed effort to seek agents effective against these bacteria. This study aimed to isolate and identify the bacterial pathogens of diabetic foot wound infection in Khartoum State and test their susceptibility to the most common antibiotics used in treating patients with diabetic foot wound compared to the activity of different concentrations of methanol extract for medicinal plant vitis vinifera leaves using Agar disc diffusion method under standard laboratory conditions. This was a descriptive cross-sectional study conducted in Khartoum State-Sudan, during the period from March 2018 to August 2020. One hundred wound swabs were collected form diabetic patients of foot infections from both gender (68% males and 32% females). Out of the hundred specimens 93(93%) showed bacterial growth, from which five types of pathogenic bacteria were isolated and identified. Fifty-three isolates were Gram negative bacteria (57%) and 40 (43%) were Gram positive bacteria. The most predominant isolate was Staphylococcus aureus 40(43%) followed by Pseudomonas aeruginosa 15(16%), Klebsiella pneumoniae 13(14%), Proteus mirabilis 13 (14%) and Escherichia coli 12(13%). The methanol extract of Vitis vinifera leaves carried out on Soxhlet extractor. It was screened for the antibacterial activity against multidrug resistant isolates and the results showed a high antibacterial activity at 100% concentration, moderate activity at 50% and low activity produced by 25 % concentration. This result authenticates the antibacterial activity of Vitis vinifera leaves and support the traditional use of the plant in therapy of bacterial infection