Antimicrobial Susceptibility and Molecular Detection of Carbapenem Resistance Genes of Proteus mirabilis Isolated from Clinical Specimens in Khartoum State - Sudan

Abstract

Carbapenems are the most powerful β-lactam antibiotics against bacteria especially multidrug resistant isolates. Since there were no comprehensive studies about prevalence of carbapenem resistance genes in Sudan, so this study was conducted to detect carbapenem resistance genes of Proteus mirabilis from clinical specimens in Khartoum State during the period from March to November 2019. A total of fifty (n=50) Proteus mirabilis isolates were included in this descriptive case laboratory-based study. P.mirabilis were isolated from urine, wound swab, high vaginal swab and semen samples which were obtained from different hospitals in Khartoum State. The isolated organisms were stored in 20% Glycerol peptone media and were inoculated on nutrient agar and MacConkey’s agar, and reidentified by using biochemical tests. Antibiotic susceptibility tests were carried out using Kirby Bauer disc diffusion technique. DNA was extracted using GenoLyse method, then were tested for the presence of carbapenem resistance genes (NDM, IMP, VIM, OXA-48 and KPC) using Multiplex PCR. Out of fifty isolates, 48(96%) were resistant to ceftazidime, 31(62%) were resistant to ciprofloxacin, 30(60%) were resistant to amikacin, 43(86%) were resistant to gentamicin and 13(26%) were resistant to imipenem. All 50 P.mirabilis isolates investigated were negative 0 (0%) for all five carbapenem resistance genes. This study concluded that, there is no carbapenem resistance genes (NDM, IMP, VIM, OXA-48 and KPC) among P.mirabilis isolates in Khartoum State.