Molecular Detection of Plasmids Mediated Colistin Resistance (MCR-1) Gene Of Already Identified Klebsiella pneumoniae Isolated from Clinical Specimens in Khartoum State, Sudan

Abstract

Antibiotic resistance is global crisis rapidly disseminated causing huge health and economic problem. Plasmid mediated colistin resistance gene (MCR-1) which has the potential to transmit horizontally between bacteria strains and specie is an important causes of colistin resistance. Fifty isolates of already identified K.pneumoniae were collected from several hospital at Khartoum State from Jun to September in 2019. For confirmation the isolates were cultured on Nutrient agar and Cystine lactose electrolyte deficient (CLED) agar to detect colony growth characteristics, biochemical tests were also performed, Antimicrobial susceptibility was done, using Kirby Bauer diffusion method. The following antibiotics were used: Amoxicillin, Ciprofloxacin, Amikacin, Ceftazidime, Imipenem and Colistin. DNA was extracted using boiling method and PCR was done for K.pneumoniae and mcr-1 gene identification usig following primer (mcr-1 F (5' CGGTCAGTCCGTTTGTTC-3') and mcr-1R (5'-CTTGGTCGGTCTGTAGGG-3')). All the isolates were confirmed as K.pneumoniae. The isolated organisms in this study were highly resistant to amikacin (90%), ceftazidime (95%), ciprofloxacin (80%) and amoxicillin (100%). There were 20 (40%) isolates resistant to colistin and 30 (60%) were sensitive. PCR amplification showed that only 1 (2%) clinical isolates carried mcr-1 gene. The results was reported the presence of mcr-1 gene in K.pneumoniae isolates