Biosynthesis and Antibacterial Activity of Silver Nanoparticles

Abstract

Recently, noble metals nanoparticles are gained the interest of the researchers due their unique physical characteristics. Among noble metals nanoparticles,silver nanoparticles exhibit sharp and distinct absorption band in the visible region of electromagnetic spectrum associated with localized surface Plasmon resonance. Therefore, the appearance of absorption band invisible region is considered as confident conformation of the formation of silver nanoparticles. In this work, silver nanoparticleswere synthesized based onTurkevich methodusing acacia nilotica extract as the reducing agent and silver nitrate as the source of silver.During the preparation process, the color of the solution was changed after adding the acacia nilotica extract, indicating the formation of silver nanoparticles. The UV-Vis spectrometer was further confirming the formation of silver nanoparticles.The effect of the concentration and heating time on the optical properties of silver nanoparticles was investigated. The obtained results showed that both concentration and heating time had no effect on the formation of silver nanoparticles but instead enhanced the reduction rate. The particle size of silver nanoparticles was calculated using Mie theory and found to be about 5nm. The antibacterial activity of the obtained Ag NPs with optimum parameters was investigated against Escheriachia coli using well diffusion method. The obtained results showed considerable effect against Escheriachia coli.