Evaluation of Glucose Level in Serum and Plasma Using Different Anticoagulants at Room Temperature

Abstract

Back ground: Glucose is key to keeping the mechanisms of the body in top working order. Along with fats, glucose is one of the body's preferred sources of fuel. Glucose levels that are unhealthy or out of control can have permanent and serious effects. Objective: To measure blood glucose level storage in different anticoagulants at room temperature. Methodology: The study was conducted in Khartoum state from the period of March to September 2020. Including 60 healthy volunteers age range between 20 to 60 years old. The blood samples were dispended into different containers (fluoride oxalate, sodium citrate, lithium heparin and plain container). The glucose level was measured initially (at zero time) after 2, 4, 6 and 8 hours in the plasma and serum using colorimetric method (glucose oxides) then the data obtained were analyzed using (SPSS) in computer program. Results: The result show that the stability of blood glucose level in fluoride oxalate up to 4 hours (P-value ≥ 0.005) while in heparin and sodium citrate and serum up to 2 hours (P-value ≥ 0.005). Conclusion: From the result it was conclude that fluoride oxalate is more suitable anticoagulant for blood glucose estimation.