Detection of Methylenetetrafolate Reductase Polymorphisms (MTHFR C677T) in pregnant women with Unexplained Recurrent Pregnancy Loss at Elgezira State in Sudan

Abstract

This was retrospective analytical case control study conducted at Elgezira state during period of April to December 2018 ,aimed to detection of Methylentetrahydrofolate reductase polymorphism (MTHFR C677T) in Pregnant Women with Unexplained Recurrent Pregnancy Loss . Total of 80 women were involved in this study, 40 of them with recurrent pregnancy loss ( cases ), and 40 women with no history of recurrent pregnancy loss (controls). 2 ml of venous blood samples were collected from each subject in EDITA blood container for PCR techniques. Data were analysis by SPSS version 16. Methylenetetrahydrofolate reductase (MTHFR C677Tpolymorphism) plays a key role in folate metabolism, MTHFR C677T poly morphism reduce enzyme activity lead to decrease conversation of homocystine to methionine subsequently result in homocystine accumulation in the blood (Hyperhomocysteinaemia), hyperhomocysteinaemia is linked to atherosclerosis resulting in arterial and venues thromboembolism. The study population were distributed according to mutation to mutant type (MTHFRC677T polymorphism) represent 37.5% among cases and 0% among controls, and wide type were 2.5% in cases and 41.3% in controls. while the heterozygous were 10% among cases, and 8.8% among controls . This subjects were distributed according to number of abortion to two times, three times, four times ,five times, and six times .The most frequent number of mutation two times and least number of mutation six times. The result show an association between number of abortion and mutation in MTHFR C677T gene polymorphism .The age groups are from 20-36 years. There is no association between age and mutation of MTHFR C677T gene polymorphism. The mutation distributed between cases and controls. In conclusion this study conducted that Methylentetrahydrofolate reductase MTHFR C677T polymorphism consider as risk factor for unexplained recurrent pregnancy loss URPL at Elgezira state in sudan.