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Antibacterial Activity of Nigella Sativa Extract against Pathogenic Bacterial Isolate

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dc.contributor.author Mnan, Hanaa Awad Alkareem Ahmed
dc.contributor.author Supervisor, - Omer Mohamed Khalil
dc.date.accessioned 2020-09-16T11:08:33Z
dc.date.available 2020-09-16T11:08:33Z
dc.date.issued 2018-01-01
dc.identifier.citation Mnan, Hanaa Awad Alkareem Ahmed.Antibacterial Activity of Nigella Sativa Extract against Pathogenic Bacterial Isolate\Hanaa Awad Alkareem Ahmed Mnan;Omer Mohamed Khalil.-Khartoum:Sudan University of Science and Technology,College of Medical Laboratory Science,2018.-50p.:ill.;28cm.-M.Sc. en_US
dc.identifier.uri http://repository.sustech.edu/handle/123456789/25039
dc.description Thesis en_US
dc.description.abstract There are many reports about usage medicinal plants and their potential as possible therapeutic agent against human pathogens. The aim of this study was to determine the antibacterial activity of different concentrations of petroleum ether, methanol and aqueous extracts of the medicinal plant Nigella sativa (seeds) using disc diffusion method on reference standard and selected pathogenic isolates of bacteria. The study was conducted in Khartoum state, Sudan, during the period from May to November 2018. Fife Reference strain and forty isolated pathogenic bacteria were tested for their sensitivity to Nigella sativa extracts. The Reference strain includes P. aeruginosa ATCC27853 K. pneumonia ATCC 53657, E. coli ATCC 25922, B. subtilis NCTC8236. While the selected pathogenic strain included Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Proteus species, staphylococcus epidermidis, bacillus cerus and Pseudomonas aeruginosa. Petroleum ether and methanol extract of Nigella sativa showed pronounced dose dependant antibacterial activity against the Reference strains and clinical isolates of Staphylococcus aureus, staphylococcus epidermidis, B. subtilis NCTC8236, bacillus cerus, E. coli ATCC 25922 and clinical isolates of Escherichia coli. But showed no activity against Klebsiella pneumoniae ATCC 53657 and Proteus species. Petroleum ether extract was partially active against the reference strain of Pseudomonas aeruginosa ATCC27853, while the isolated pathogenic strains of Pseudomonas aeruginosa were non sensitive to the extract. The aqueous extract showed no activity to all tested Grams negative organism it just showed activity against Staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis NCTC8236 and bacillus cerus. The study concluded that the greatest activity of Nigella sativa extract were against Gram positive bacteria. Further studies using large sample size and advance techniques are required to validate the results of this study. en_US
dc.description.sponsorship Sudan University of Science & Technology en_US
dc.language.iso en en_US
dc.publisher Sudan University of Science and Technology en_US
dc.subject Antibacterial Activity en_US
dc.subject Nigella Sativa en_US
dc.subject Pathogenic Bacterial en_US
dc.title Antibacterial Activity of Nigella Sativa Extract against Pathogenic Bacterial Isolate en_US
dc.title.alternative النشاط البكتيرى المضاد لمستخلص الحبة السوداء على العزلات البكتيرية الممرضة en_US
dc.type Thesis en_US


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