Molecular detection of Methicillin Resistance in Staphylococcus aureus Isolated from raw cow milk in Ghebaish Locality,West Kordofan

Abstract

The occurrence of MRSA organisms is one of the problems that facing the world now in treatment of bacterial infections. The antibiotic resistance is common among pathogenic bacteria associated with both community acquired and nosocomial infections, Staphylococcus aureus is notorious for its ability to become resistant to antibiotics. The aim of this study was to detect the specific mecA gene in the Staphyllococcus aureus isolates. Staphylococci strains were isolated from raw cow milk in Ghebaish Locality and were identified with conventional methods and APi system. A total of 200 samples were collected and 33 Staphylococci strains (16%) isolates were obtained. Bacterial DNA was extracted from each isolate using boiling method. PCR was used to detect mecA gene. The results indicate that 23 isolates were positive to mecA gene (69%).The mecA gene sequence of S. aureus showed high similarities with those recorded in gene bank. The study concluded that, Bacterial DNA extracted from Staphylococcus aureus reflected appearance of mecA gene which was responsible of resistance to methicillin.