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Isolation and Molecular Identification and Sequencings of Nocardia and Nocardia- like spp Isolated from Soil and Milk of Goats, Sheep, Cattle and with Mastitis in Khartoum Stats

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dc.contributor.author Mohamed, Tagreed Idrees
dc.contributor.author Supervisor, -Galal Eldin Elazhari Mohammed
dc.contributor.author Co-Supervisor, -Mogahid El Hassan
dc.date.accessioned 2019-05-15T09:12:45Z
dc.date.available 2019-05-15T09:12:45Z
dc.date.issued 2019-01-24
dc.identifier.citation Mohamed, Tagreed Idrees . Isolation and Molecular Identification and Sequencings of Nocardia and Nocardia- like spp Isolated from Soil and Milk of Goats, Sheep, Cattle and with Mastitis in Khartoum Stats \ Tagreed Idrees Mohamed ; Galal Eldin Elazhari Mohammed .- Khartoum:Sudan University of Science & Technology,College of Veterinary Medicine,2019.- 105 p.:ill.;28cm.-Ph.D. en_US
dc.identifier.uri http://repository.sustech.edu/handle/123456789/22630
dc.description Thesis en_US
dc.description.abstract This work was intended to investigate the role of Nocardia and Nocardia -likeorganisms as causative agents of mastitis in soil sample and milk sample from goats ,cow and sheep. It was carried out at different farms in Khartoum Stat. A total of three hundred milk samples of cow ,sheep and goat and twenty soil samples were collected from the same farms in different sites for isolation and identification of Nocardia and Nocardia —likeorganisms. All milk samples were cultivated onto Tryptic Soya Agar (TSA) ,and soil cultured on to Tryptic Soya Agar medium (TSA) supplemented with a combination of tetracycline (5mg/ml) and nystatin (50 mg/ml). All isolates 11(11%) from milk of goats,13(13%) from milk of cow and 7(35%)from soil sample were identified phenotypically as Nocardia sppby cultural, morphological, and biochemical tests (urease test, catalase test and degradation of xanthine, tyrosinc, casein, sugar fermentation , growth at 45°C), mycolic acid content and antimicrobial susceptibility test. The isolates were tentatively identified as member of the genus Nocardia on the morphological, biochemical and mycolic acid pattern. Comparative analysis of the 16S RNA gene sequencing confirm that the isolates fall within the phylogenic branch whichaccommodates member genus Mycobacteria, Dietzia spp and Rhodococcusspp. In this study we consider the recognition of Nocardia and Nocardia-like based on phenotypic tests was strenuous, but definitive identification was attainable by molecular methods. en_US
dc.description.sponsorship Sudan University of Science & Technology en_US
dc.language.iso en en_US
dc.publisher Sudan University of Science and Technology en_US
dc.subject Veterinary Medicine en_US
dc.subject Microbiology en_US
dc.subject Isolation and Molecular Identification en_US
dc.subject Sequencings of Nocardia and Nocardia- like spp en_US
dc.subject Isolated from Soil and Milk of Goats, Sheep en_US
dc.title Isolation and Molecular Identification and Sequencings of Nocardia and Nocardia- like spp Isolated from Soil and Milk of Goats, Sheep, Cattle and with Mastitis in Khartoum Stats en_US
dc.title.alternative العزل والتعريف التسلسلي لبكتريا النوكارديا وأشباه النوكارديا من عينات التربة ولبن الماعز والأغنام والأبقار المصابات بالتهاب الضرع في ولاية الخرطوم en_US
dc.type Thesis en_US


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