Immunohistochemical Detection of Cyclin D1 in Thyroid Tumorsamong Sudanese Patients

Abstract

This is a hospital based analytical case control study was conducted at Omdurman Teaching Hospital during the period July to September 2017. The study aimed to detect Cyclin D1 in thyroid tumors among Sudanese patients. A total of 40 formalin fixed paraffin blocks previously diagnosed as thyroid tumors were selected. 17 (42.5%) samples were papillary thyroid carcinoma , 9 (22.5%) were follicular thyroid carcinoma, 2 (5%) were medullary thyroid carcinoma, 2 (5%) were anaplastic thyroid carcinoma and 10 (25%)samples were benign thyroid tissue (multinodular goiter), one section of 3μm thickness was cut by rotary microtome from each block and stained by immunohistochemical method (avidin-biotin immune technique) for Cyclin D1detection. The data obtained was analyzed using SPSS computer program version 16. Patients age ranged between 11 to 65 years with mean age 37 years, most of the patients were less than 45 years. The sex of the patients revealed that 10 (25%) were males and 30 (75%) were females.

Positive expression of Cyclin D1 was detected in (24/30) of malignant samples, while (6/30) samples showed negative expression, and (8/10) of multinodular goiter samples showed negative expression for cyclin D1, and (2/10) samples were positive. This result showed significant association between expression of cyclin D1 and type of thyroid tumors (P.value=0.001). The study concludes that the positive expression of Cyclin D1 is associated with malignant thyroid tumors rather than benign tumors.