Comparison of Some Biochemical and Molecular DNA Extraction Techniques Used in Sudan Criminal Laboratory

Abstract

The advantages of DNA typing and its use for human identity testing has revolutionized law enforcement investigations in recent years by allowing forensic laboratories to match suspects with minuscule amounts of biological evidence from a crime scene. Equally important is the use of DNA to exclude suspects who were not involved in a crime or to identify the rapist in sexual crimes and in paternity cases from different samples (Evidences) from different cases were collected and extracted, amplified and analyzed for the 16 loci, and their forensic applicability were evaluated. Results were obtained by using different methods for DNA extraction. For detection of fluorescently labeled amplified products, the ABI Prism® 310 Genetic Analyzer was used. STR loci to provide a rapid, sensitive, and reliable method of DNA typing for forensic identification. Thirty five different cases from inside and outside of Khartoum state were used. Fifteen Paternity cases, child’s DNA were compared to their mothers, and the alleged father to identify child’s, father, eleven cases were confirmed by their father to the child (inclusion), and four cases were denied their father (exclusion). .Ten Murder cases, the suspect’s DNA profile were compared to some evidences collected from the crime scene, which led to identify the criminal, and link between victim’s DNA on object in suspect’s possession, Ten Rape cases were compared to suspect’s DNA on victim, victim’s body or clothes, five cases were matched (inclusion), four were not matched (exclusion) and one was contaminated.