SUST Repository

Detection of Tissue Factor Pathway Inhibitor-2 Gene Mutation and its Correlation with Coagulaton Parameters in Sudanese Pediatric Patients with Leukemia

Show simple item record

dc.contributor.author Idris, Eihab Idris Mohammed
dc.contributor.author Supervisor, - Babiker Ahmed Mohamed
dc.contributor.author Co-Supervisor, - Fath Elrahman Mahdi Hassan Gameel
dc.date.accessioned 2017-12-07T10:49:26Z
dc.date.available 2017-12-07T10:49:26Z
dc.date.issued 2016-01-01
dc.identifier.citation Idris, Eihab Idris Mohammed.Detection of Tissue Factor Pathway Inhibitor-2 Gene Mutation and its Correlation with Coagulaton Parameters in Sudanese Pediatric Patients with Leukemia\Eihab Idris Mohammed Idris;Babiker Ahmed Mohamed.-Khartoum:Sudan University of Science & Technology,Medical Laboratory Science,2016.-107p.:ill.;28cm.-Ph.D. en_US
dc.identifier.uri http://repository.sustech.edu/handle/123456789/19301
dc.description Thesis en_US
dc.description.abstract This is analytical case control study was carried out in radio isotope center Khartoum (RICK) to detect TFPI-2 gene mutation and its correlation with coagulation parameter in Sudanese pediatric patients with leukemia. Ninety-eight patients less than 15 years with mean age for all (9.03 ± 4.058) where diagnosed with leukemia of both gender 52 (53.1%) were males and 46 (46.9%) were females .The center administration and the relatives of the patients were informed about the study and agreement of participation was obtained. In additive ninety-seven healthy children, less than 15 years 53 (54.6%) were males and 44 (45.4%) were females with mean age for all (8.08 ± 4.033) selected as a control group. A structured questionnaire used to collect information about patient age, sex, and patient history of disease. Five ml of venous blood was collected, three ml in EDTA and 1.8 ml in trisodium citrate anticoagulant container from all patients and controls. Reverse transcription polymerase chain reaction followed by restriction-fragment length polymorphism (RFLP) analysis of the TFPI-2 was, carried out for all patients and controls samples. TFPI-2 gene was investigated in all samples, there were 14(14.3%) of the patients group have mutation, 10(71.4%) of them were homozygous and 4 (28.6%) were heterozygous. No participant among control group (0%) had TFPI-II gene mutation; there was significant association between patients, which have TFPI-2 gene mutation, and control group, P value 0.0258. Automated hematological analyzer (Sysmex KXN-21) used to measure platelet count for the patient and control group. Automated coagulation tests (PT, PTT, fibrinogen, protein C and D-dimer level) were measured using coagulation analyzer (sysmex CA 500) for all patient and control group. Statistical package for social science (SPSS) computer program was used for data analysis In patients group with TFPI-2 gene mutation the result of PT mean level was (17.05 ± 2.974). PTT mean level was (39.564 ± 6.809), fibrinogen mean level (2.759 ± 0.630), protein C mean level (0.845± 0.198) and platelet count mean (288.21 ± 155.797), while in the patients group without mutation the result of, PT mean was (17.689 ± 6.741), PTT mean was (40.555 ± 11.551), fibrinogen mean (2.659 ± 0.681), protein C mean level (0.822 ± 0.037) and platelet count mean was(289.67 ± 136.85). There were insignificant variations in coagulation tests parameter (platelet count, PT, INR, APTT, protein C and fibrinogen) between patients with TFPI-2 gene mutations and those without TFPI-2 gene mutations. There were only two patients had high D-dimer level which excludes disseminated intravascular coagulation. en_US
dc.description.sponsorship Sudan University of Science and Technology en_US
dc.language.iso en en_US
dc.publisher Sudan University of Science & Technology en_US
dc.subject Tissue Factor en_US
dc.subject Pathway Inhibitor-2 en_US
dc.subject Gene Mutation en_US
dc.subject Coagulaton Parameters en_US
dc.subject Leukemia en_US
dc.title Detection of Tissue Factor Pathway Inhibitor-2 Gene Mutation and its Correlation with Coagulaton Parameters in Sudanese Pediatric Patients with Leukemia en_US
dc.title.alternative الكشف عن الطفرات الوراثية لمثبط مسار العامل النسيجى -2 المانع للتخثر وعلاقته باختبارات التجلط لدي الأطفال السودانيين المرضى بسرطان الدم الأبيض en_US
dc.type Thesis en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Share

Search DSpace


Browse

My Account