Determine the Relationship between ABO Blood Groups and Von Willebrand Factor Antigen Level Among Healthy Donors in Khartoum State

Abstract

This is observation descriptive cross sectional study was carried out to study the Relationship between ABO blood groups and von Willebrand factor antigen level among healthy Donors was conducted in National Public Health Laboratory (Central Blood Bank) in Khartoum state during the period from January 2017 to May 2017. The study included sixty samples from healthy volunteer blood donors ,1.8ml of venous blood was with drown from each healthy donors, placed in tri sodium citrate containerthen separate the sample by centrifuge and extract plasma to measure von willebrand factor antigen level using Enzyme Linked Immunosorbent Assay. For each specimen ABO and RhD antigen were already determined and the personal data was recognizing by questionnaire. The results was analyzed by statistical package for social science computer program (SPSS) version 16.the results obtained from healthy donors showed that the mean of von willebrand factor antigen level within different ABO blood groups is (0.741 IU/ml) in blood group A, (0.573 IU/ml) in blood group B, (0.913 IU/ml) in blood group AB and (0.425 IU/ml) in blood group O. The results showed there was statistically significant differences of mean vWF:Ag within different ABO blood groups (P. value 0.000)that subjects with non-O blood group had a level significantly higher than those of group O, plasma vWF levels were lowest in group O (mean vWF:Ag 425 IU/ml) and highest in group AB subjects (mean vWF:Ag 913 IU/ml) while there was no statistically significant difference between the plasma vWF: Ag concentration and Age (P. value 0.077) and there was no statistically significant difference between the plasma vWF: Ag concentration and RhD Antigen (P. value 0.583) .