Immunohistochemical Detection of Cytokeratin 19 in Thyroid Tumors

Abstract

This is retrospective study aimed to detect the expression of CK19 tumor marker in thyroid tumor using immunohistochemistry. The study was conducted in Omdurman Military Hospital and Omdurman Teaching hospital during the period from January to September2017.Forty paraffin embedded tissue blocks previously diagnosed as thyroid tumors were selected.The patients information was collected from the files of patients. The patients ages ranges between 19-82 years, with mean age40 years. Tissue sections were cut and stained using dextrane-polymer technique for immunohistochemical detection of ck19tumor marker .The obtained data were analyzed using SPSS computer program,frequency, mean and chi-square value were calculate .Out of 40 patients with thyroid tumors, 30 (75%) were females,while 10(25%)were males. Regarding the histological diagnosis the 20malignant cases were 9,7,3,1,papillary carcinoma, follicular carcinoma , medullary carcinoma and anaplastic carcinoma respectively. and the 20 benign lesions were 10goiter and 10 follicular adenoma. Ck19 showed positive result in 7,2,1,0,2,1samples in papillary carcinoma, follicular carcinoma, medullary carcinoma, anaplastic carcinoma, goiter, follicular adenoma respectively,with significant relation between ck19 expression and histopathologicaldiagnosis p.value:0.04.The study concluded that the ck19 tumor marker is highly expressive marker in thyroid carcinomas.