Extraction and Analysis of Hydnora abyssinica Root (Tartose)

Abstract

The aim of this study was to determine the composition of hydnora abyssinica root extract sample, from chemical and antibacterial site of view. The root sample was extracted by absolute methanol (99.8%). X-Ray fluorescence technique was used for determination of some minerals. The analysis showed the presence of calcium Ca, magnesium Mg, sodium Na, potassium k, aluminum Al, phosphors P, zinc Zn, nickel Ni, copper Cu ,iron Fe , sulphur S, rubidium Rb , silicon Si, chloride and bromide. Infrared spectroscopy technique was used for main functional group occurrence. This showed the presence of O-H, C-O, C=C, C-H, benzene ring). The agar well diffusion method was used to determine the susceptibility of bacterial strains to the extract of the plant. The antimicrobial activity of Hydnora abyssinica against some types of bacteria (Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa Bacillus subtilis) demonstrated a mean zonediameter of inhibition ranging from 12 to 22 mm and that mean, the extract is active against bacteria. Ash content was determined and it was found to be 3.81%