Isolation and Identification of Camelpox Virus in Eastern Sudan

Abstract

In this study, skin biopsies were collected from camel herds in Eastern Sudan showed signs suggestive to camelpox, for histopathology, virus isolation and identification. The affected camels were febrile, depressed, prostrated and anorexic. Also edema of face and head and lesions involved the whole head, neck, buttock, abdomen, chest, legs, ears, udder and tail.Histopathology results of the skin biopsies revealed marked degree of acanthosisand ballooning (hydropic) degeneration and vacuolation of acanthocytes.nBothHyperkeratosis and thedermal layer infiltrated with inflammatory cells, with dermal fibroplasia. Virus isolation succeeds on CAM and Vero cells. The isolates were identified as camel pox by AGPT and VNT and PCR. PCR was considered the rapid and sensitive for the diagnosis of Camelpox.