Detection and Isolation of Bovine Parainfluenza Virus Type 3 (PIV-3) in Cattle in North Kordofan State, Sudan

Abstract

Bovine parainfluenza virus type3 (BPIV-3) is a virus that causes respiratory infection in cattle worldwide. A total of 100 lung specimens condemned due to pneumonia were collected from Elobied slaughter house at North Kordofan state. Sandwich ELISA was used to detect BPIV-3 antigen in lung tissue specimens, Vero cells were used to isolate PIV-3 virus. Direct immunoflourcent antibodies test (FAT) was used to confirm the ELISA positive results of BPIV-3 antigen in lung tissue specimens as well as for the identification of the isolated virus. PCR was used for detection of BPIV-3 genome. PIV-3 antigen was detected in 20% of the 100 cattle lung tissue specimens tested by ELISA while it was detected in 25 (25%) cattle lung specimens tested by FAT. Using FAT, all ELISA positives for PIV-3 and 5 of the 80 ELISA negative specimens were found to be positive. One sample was isolated in Vero cells. PIV-3 genome was detected in all samples tested by PCR.