Detection of Methicillin-resistant Staphylococcus aureusin Stethoscopes

Abstract

This is a laboratory based study, carried out during the period from June to November,2014. The study objective was to detect Methicillin-resistant Staphylococcus aureus(MRSA) in stethoscopes in Khartoum State hospitals. The bacterial isolates under assessment (n = 14) were obtained from the Research Laboratory, Sudan University of Science and Technology. The isolates were cultured on nutrient agar by streaking to check their purity. Gram's stain and biochemical tests were used to confirm the identification of the isolates. Antimicrobial susceptibility test was carried out by modified Kirby-Bauer disk diffusion method.E-test was done to determine Minimum Inhibitory Concentration (MIC). MRSAweredetected by their resistant Oxacillin. The results showed that all identified isolates (n = 14) were Staphylococcus aureus, study of antimicrobial susceptibility revealedthatallStaphylococcusaureus were resistant to Oxacillin. The study concludedthatMRSA are common in hospitals. Further studies are required with large numbers of isolates to validate the results of this study.