dc.contributor.author |
Mohammed, Huda Abdul Gaffar |
|
dc.contributor.author |
Supervisor, - Sulieman Mohamed El Sanousi |
|
dc.date.accessioned |
2013-09-02T06:08:26Z |
|
dc.date.available |
2013-09-02T06:08:26Z |
|
dc.date.issued |
2012-01-01 |
|
dc.identifier.citation |
Mohammed,Huda Abdul Gaffar.Effect of Cold Shock on Growth and Antibiotics Susceptibility Patterns of Staphylococcus aureus, Klebsiella pneumoniae and Shigella dysenteriae/Huda Abdul Gaffar Mohammed;Sulieman Mohamed El Sanousi.-khartoum:Sudan University of Science and Technology,Medical Laboratory Science,2012.-60p. : ill. ; 28cm.-M.Sc. |
en_US |
dc.identifier.uri |
http://repository.sustech.edu/handle/123456789/1402 |
|
dc.description |
Thesis |
en_US |
dc.description.abstract |
This work was carried out to study the effect of cold shock on the growth of
Staphylococcus aureus, Shigella dysentiea and Klebsiella pneumoniea moreover;
evaluation of minimum inhibitory concentration of amoxicillin, cephalexin,
ceftriaxone, ciprofloxacin, chloramphinicol and co-trimoxazole antibiotics with
these organisms before and after cold shock.
Serial dilutions were performed from an overnight nutrient broth for s.aureus,
s.dyseteriae and k.pneumoniae. The diluted sample was placed on the center of a
solid cooled nutrient agar medium and then spread. After incubation at 37 ̊c over
night the colonies were counted each bacterium represent a colony forming unit
(CFU). The colonies were counted according to Mils and Misra (1938) method, this
method was done before and after cold shock. The results of counting for s.aureus,
s.dysenteriae and k. pneumoniae is decreased after cold shock than before cold
shock that indicate the cold shock is affect on bacterial growth.
For MIC plates of Mueller Hinton agar were inoculated will bacterial suspension of
shocked and non shocked bacteria different concentrations of MIC test strips
HiComb from range A and B of (amoxicillin, ceftriaxon, cephlexine, ciprofloxacin,
chloramphinicol and cotrimoxazole) were applicated after 24 h of incubation the
MIC was determined from the highest dilution of the antibiotics showing no
growth, the results for s.aureus with amoxicillin, ceftriaxon, cephlexine,
chloramphinicol and cotrimoxazole before and after cold shock are not different but
with ciprofloxacin was different before cold shock 7.5mcg and after 3mcg. Results
of s.dysenteriae with amoxicillin, ceftriaxon, ciprofloxacin, chloramphinicol and
cotrimoxazole were not different but with cephalexine before cold shock 30mcg and
after cold shock 60mcg it’s become more resistant after cold shock. The MIC before
and after cold were not different except in some cases. |
en_US |
dc.description.sponsorship |
Sudan University of Science and Technology |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Sudan University of Science and Technology |
en_US |
dc.subject |
Cold Shock |
en_US |
dc.subject |
Staphylococcus aureus |
|
dc.subject |
Klebsiella pneumoniae |
|
dc.subject |
Shigella dysenteriae |
|
dc.title |
Effect of Cold Shock on Growth and Antibiotics Susceptibility Patterns of Staphylococcus aureus, Klebsiella pneumoniae and Shigella dysenteriae |
en_US |
dc.type |
Thesis |
en_US |