Detection of Multi-drugs Resistance among Bacteria Isolated from Computers’ Keyboards

Abstract

This is a laboratory-based study, carried out during the period from May to November, 2014. The objective of this study was to detectmulti-drug resistance among bacteria isolated from computers' keyboards. The isolated bacteria under assessment were obtained from the Research Laboratory, Sudan University of Science and Technology. Gram's stain and biochemical tests were used to confirm the reidentification of the isolates. Multi-drug resistance among the isolates was detected by modified Kirby-Bauer disk diffusion method. The antibiotics assessed were Amikacin, Gentamicin, Amoxi-clav,Cotrimoxazole,Ciprofloxacin, Erythromycin, Ampicillin, Amoxicillin, Tetracycline, Penicillin, Meropenem, Norfloxacin, Cefuroxime, and Cloxacillin. The results showed that the reidentified isolates werePseudomonas species 38(38%), S. aureus22 (22%),S. epidermis16(16%),E. coli12(12%),Klebsiella species8(8%) and S. haemolytics4 (4%). The study concludedhat the result of multi-drug resistance among Gram-negative bacteria was very low. While the multi-drug resistance among Gram-positive bacteria (Staphylococcus species) showed that 100% of S. epidermiswere multi-drug resistant to Gentamicin, Cotrimoxazole,Erythromycin, Tetracycline and Cloxacillin.