Detection of CDX2 Tumor Marker and Human Papilloma Virus in Esophageal Tumors

Abstract

This is a descriptive retrospective study conducted at Ibn Seina hospital and Sudan University of Science and Technology during the period from April 2014 to October 2014. The study aimed at detecting CDX2 and association of Human Papilloma Virus (HPV) in esophageal tumor among Sudanese patients using immune-histochemistry and polymerase chain reaction. Samples from 30 patients previously diagnosed with esophageal tumor (20 with esophageal cancer and 10 with benign). Their ages ranging between 8 to 82 years with mean age 37. From each block two slides were cut one for CDX2 and other for HPV detection, also 10µ was cut from each block in eppendorf tube for detection of HPV type 18 gene using PCR.SPSS version 16 computer program was used to analyze the data. Mean, frequency and Chi square were calculated. From thirty patients twenty were male (66.7%) and ten were female (33.3%). CDX2 was detected in four (13.3%) and twenty six samples (86.7%) were show negative result. Seven cases (23.3%) were positive for Human papilloma virus and 23 (76.7%) were negative by using immune-histochemistry. The detection of HPV type 18 was done for 7 samples by using PCR only 5 samples were gave positive result. On the basis of these findings the study concluded that, there is no association between CDX2 expression and esophageal cancer (P= 0.129). There is no relation between HPV expression and esophageal cancer (P= 0.222).