Abstract:
This cross sectional hospital based study was carried out in Khartoum
and Omdurman teaching hospitals to determine the effect of Entameba
histolytica on hemoglobin level and total white blood cell with
differential counts. In period between 10\5\2007 to 29\7\2007. The total
sample size equal 150,were randomly selected from Sudanese patients
of (77 males and 73 females), at different age groups, with and without
clinical symptoms of intestinal amoebiasis ,they were divided in to three
groups according to the questionnaire and microscopical stool
examination (wet preparation and concentration techniques),these are
non infected, infected before, and infected groups, other parasitic
infections were excluded. Subsequently 150 blood samples were
collected and analyzed using colorimetric method (0.02 ml capillary
blood added to 4ml of drabkins solution)for haemoglobin measurement,
white blood cell count using(0.02 ml of blood added to 0.38 ml of
2%Glacial acetic acid solution using Neubauer chamber for cell
count).leishman stain for differential leukocytes count was added to a
fixed thin blood film for 3-4 min then double volume of leishman buffer
was added for 6-8 min.
Haemoglobin level was found to be 12.12 g\dl, 12.08 g\dl and
11.25 g\dl in non infected, infected before and infected groups with
E.histolytica respectively. The haemoglobin level decreased in infected
group with E.histolytica and show highly significant differences
between non-infected and infected group at p<0.01 level. Mean
hemoglobin concentrations were found to be higher in males in all
groups than in females, (12.6±.5g/dl[std=1.3]versus 11.2±.5
g/dl[std=1.2],p=.002).statistically significant at p<0.01 level.
Mean leukocytes count in E.histolytica infected group (6300
c\cumm3), infected before (5300 c\cumm3) and non infected(4800
c\cumm3). When these results were compared, E.histolytica infected ,group had higher leukocytes count which exceed the normal mean value
.there is very highly significant differences at p<0.01 level
Differential leukocytes counts were increased in E.histolytica
%09.1/%88.06(,(infected group in neurophils and eosinophils count
Comparing to non-infected group(57.36%/0.92%),statistically significant
at p<0.01 level. while lymphocytes, basophiles and monocytes count in
E.histolytica infected group: (30.98%/6.06%/0.16%), were found to be
.within the normal range
