Abstract:
This study was carried out in the period from May 2007 - March 2008 to detect
pathogenic Yersinia enterocolitica from stool specimens by real-time polymerase chain
reaction technique as an alternative to traditional culture methods. Forty six stool
specimens were collected from patients attended Khartoum Teaching Hospital,
Omdurman Teaching Hospital and Gaafer Ibni-ouf Specialized Hospital.
Bacterial DNA was extracted from each stool specimen using Phenol-Chloroform
technique. Real-time PCR detect the presence of Y. enterocolitica in stool specimens, the
result revealed that only five (10.9%) specimens were positive.
The study concluded that the real-time PCR technique facilitates the detection of
pathogenic Y. enterocolitica from stool specimens without bacteriological culture.
