Abstract:
This study was aimed to detect rubella virus antibodies (IgM and IgG) by ELISA and then was confirmed by detection viral genome by Reverse transcriptase polymerase chain reaction (RT-PCR) among apparently healthy pregnant women in Khartoum State. In this study 358 plasma and sera were collected; from which 2 (0.56%) were IgM +ve and 320 (89.39%) were IgG +ve, and there was no association between IgM and IgG results with the trimester and gravidity. The results showed that; there was no association between IgM and family members, season and socioeconomic situation while IgG had no association with miscarriage and number of miscarriages.Out of 169 there was 4(2.4%) positive by RT-PCR and there was no association between residence, trimester and season of year with the presence of RV. Also it found that primagravida had a risk in acquiring infection but family size and socioeconomic situation had no risk to get infection. The current study concludes that; rubella virus genome and recent infection were detected in few apparently healthy pregnant women, while the percentage of previous infection was high.
