Abstract:
In the present work, the stems of Trianthema portulacastrum and Phoenix dactylefera pollens were extracted with 95% ethanol at ambient temperature. Qualitative tests on alcoholic extract of Trianthema portulacastrum stems revealed the presence of flavonoids, alkaloids, tannins, saponins, steroids, carbohydrates and glycosides.
The crude extracts of Trianthema portulacastrum and Phoenix daclylefera were initially purified by column chromatography. Further purification was accomplished by TLC technique. In this way Trianthema portulacastrum gave two flavonoids (compounds I and II) while Phoenix dactylefera gave compounds III and IV. The structures of these compounds were deduced on the basis of their spectral data (UV, 1HNMR and MS). The isolated compounds were assigned the following partial structures:
V
Different fractions (ethanol, chloroform, ethyl acetate and n- butanol) of the Trianthema portulacastrum stems and Phoenix dactylefera pollens were assessed for antimicrobial activity against six standard pathogenic bacteria: Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger and Candidia albacans.
For Trianthema portulacastrum all fraction, except that of ethanol, showed good antifungal activity. All fractions ,except that of n-butanol, exhibited good activity against Bacillus subtilis.
For Phoenix dactylefera, the ethyl acetate and n-butanol fractions showed excellent activity against Bacillus subtilis and the yeast Candidia albacans respectively. Also the butanol fraction gave good activity against Pseudomonas aeruginosa, Aspergillus niger and Bacillus subtilis.
