Abstract:
No wonder that grape fruit and orange are more popular fruits in Sudan. They are well
known for their nutritional and medicinal properties, but for seeds oil of two plants fruit
there is no enough studies reflecting the properties and benefits of them. This research
aims to characterized and identify the properties and components of grape fruit and
orange seeds oil. As the chemical analysis is important in formulating and developing
new products and identifying the source of problems with unacceptable product in this
work, phytochemical analysis, anti microbial activity, and physical properties were
studied to identify the grape fruit and orange seeds oil. The Sudanese grape fruit (citrus
pardisi) and orange (citrus sinensis) were collected from Algabraab field in November,
2014 the seeds were separated manually, dried at sunlight and grind. The oil was
extracted by solvent extraction method using petroleum ether, a pale yellow colored oil
(36.248)% for grape and(27.71)% for orange was obtained and characterized by physical
properties and GC-Mass analysis . The crude extractions were obtained by solvent
extraction method used methanol the yields percentage were (8.65) %for grape and
(7.81)%for orange.
The phytochemical screening was carried out using the methods described by
Martinez & Valencia, (1999), Sofowora, (1993), Harborne, (1984) and Wall et.al, (1952)with some modifications. The TLC analysis of crude extract proved the presence of many
chemical compounds. Antimicrobial activity which determined by disc diffusion method
carried for all extractions showed good results.
Fatty acids component like palmatic acid, linoleic and oleic and other compounds were
found in the two oil samples. The physical properties results gave the grape fruit and
orange seeds oil the vegetable oil behavior (can be used as edible oil), economic value
health benefits and cosmetics manufacturing uses. The presences of saponin, alkaloids,
tannins, favonoides, sterols, triterpens and cumarins in showed by phytochemical
screening consider that the two planst seeds are active against bacteria and fungi
