Abstract:
Infection with Helicobacter pylori has been associated with
the pathogenesis of chronic active gastritis, gastric and
duodenal ulcer disease. Detection of immunoglobulin G
antibodies to H. pylori by an immunochromatographic test
(ICT) offers a simple alternative to direct detection of the
organism in biopsied tissue by culture or histopathological
methods. The aim of this study was to compare the rapid
flow-through membrane-based enzyme immunoassay for
the detection of human immunoglobulin G antibodies to H.
pylori with H. pylori antigen detection in human stool.
Clinical evaluations were performed with 150 patient
serum and stool samples obtained from Yastabshiroon
Hospital. Stool samples were obtained by collection in
clean dry container at the same time as the serum
samples; were serologically categorized for the presence
or absence of H. pylori antigen. sensitivity and specificity
for this rapid enzyme immunoassay were 98% and 91%,
respectively, compared directly with antigen detection
results.
These
results
immunochromatographic
indicate
test
(ICT)
that
for
this
rapid
detection
of
immunoglobulin G antibodies is a useful technique to
determine H. pylori infection status and is a viable
alternative to invasive endoscopic procedures.
