Assessment Of Alkaline Phosphatase Activity Among Vitamin D Deficient Hypertensive Patients – Khartoum State

Abstract

Alkaline phosphatase is a group of glycoprotein enzymes that act as phosphotransferases by hydrolyzing various types of monophosphate bond at alkaline pH, and used as marker of cholestasis in hepatocellular disease. There is association between vitamin D and damage of tissue accordingly the study aim to evaluate prediction value of alkaline phosphatase activity as early marker for cholestasis in vitamin D deficient hypertensive patients A descriptive cross-section study carried out in different hospitals, eighty eight hypertensive patients were enrolled in this study 46 male and 42 female classify based on vitamin D results into three groups, group one normal vitamin D (>30 ng/ml) considered as control, group two vitamin D deficient (20 -30 ng/ml) and group three sever deficient vitamin D (<20 ng/ml). Alkaline phosphatase activity was measured kinetically and ΔA/min was calculated using spectrophotometer and vitamin D levels were estimated using competitive ELISA. Statistical analysis tests ANOVA, t-test done by using SPSS version 14. Results of frequency showed gender variation are approximately equal (1:1)ratio male 52.3% and female 47.7%, also vitamin D deficient more common in female than male (81.09% female, 45.60% male), there was no association between BMI and vitamin D deficient female in contrast male with overweight male tend vitamin D deficient, ANOVA results showed there was no significant difference between ALP activity in both group of vitamin D deficient(D deficient and sever D deficient) when compare with normal vitamin D (P-value 0.215,P-value 0.575)respectively, in contrast overweight patients showed significant increase ALP activity. Study concludes that, no change in ALP activity in vitamin D deficient hypertensive patients, and overweight subject tent to have increased ALP activity.