Comparison between Two Different Methods (Caraway Chemical and Uricase Enzymatic) for Estimation of Uric Acid in Plasma

Abstract

This study conducted during the period of ( February 2014 – August 2014) and aimed to compare between the old caraway chemical method and enzymatic method which are commonly used available uricase enzymatic method which are commonly used for measurement of uric acid in practice in sudan. This study include 50 samples collected from 50 volunteers who attended randomly. All samples collected in heparin containers and separated immediately. Venous blood samples were taken from the study population and uric acid was measured by chemical and enzymatic methods. All results were analyzed using SPSS program. These study showed that there was statistically significant increase in the results of uric acid obtained by using uricase enzymatic method (mean=3.9mg/dl) than the results obtained by using caraway chemical method (mean=3.3mg/dl) with (P.value=0.027) This study also reveal that there was statistically significant increases in uric acid levels (mean=4.4mg/dL) in males (n=24) , when compared with that of (n=26) females (mean=3.3mg/dL) with (P.value=0. 001) by uricase enzymatic method and (mean=3.8 mg/dl) in male (n=24) when compared with that of (n=26) females (mean=2.9 mg/dl) with (P.value = 0.005) by caraway chemical method. The uricase enzymatic and caraway methods can be recommended to use in sudan for uric acid measurement considering the following factors:Availability, cost, time consumed, which one easy to perform and reference value of each one