Isolation and Identification of Bacteria that Cause Contamination in Operating Theaters

Abstract

This study aims at isolation and identification of bacteria contaminating operating theaters in Khartoum Teaching Hospital (gynoclogical and pediatric surgery ). Fifty swabs samples were obtained from different sites in operating theater.(bed,trolleis,floor,air,operating lamps). The isolation was done by using MacConkey’s and blood agar,and streaking technique was employed for the isolation and purification of the colonies. The identification was done by using Gram’s stain and biochemical tests.The study reveals that 44% of operating theaters were contaminated with bacteria. The isolates were Pseudomonas aeruginosawas the most common isolate (63.6%), followed by Micrococci.spp (22.7%),Bacillus . spp(9.2%), and Staphylococcus. aureus(4.5%)