Prevalence of Antimicrobial Residues and Detection of Aflatoxin M1 in Dairy Milk in Khartoum State

Abstract

This study was conducted to investigate the level of two antibiotic residues (Neomycin and Tylosin) in dairy milk by Enzyme- Linked Immuno Sorbent Assay, and to detect and evaluate the levels of Aflatoxin M1 in raw dairy milk by VICAM Flurometer in Khartoum state. In 64 milk samples the presence of Neomycin and Tylosin were detected in all collected samples (100%) in high concentration levels. In 50 raw dairy milk samples the presence of Aflatoxin M1 were detected with high concentrations in 49 (98%) of detected samples. The results were higher than the maximum tolerance limit (50 ng/l) accepted by FDA, Codex Alimentarius Commissions (CAC), and European Union countries. Statistical evaluations showed 95% confidence interval with significant differences between the three detected districts (P = 0.05) for neomycin as well as tylosin. The high mean of Tylosin in (Khartoum: Mean ± STD=0.79 ± 0.76, (Khartoum north: Mean ± STD=0.69 ± 0.70) and Omdurman (: Mean ± STD=0.82 ± 0.88) respectively. and in Neomycin The high mean in (Khartoum: Mean ± STD=3.43±0.29, (Khartoum north: Mean ± STD= 3.010±3.22) and Omdurman (Mean ± STD=2.10±0.34) respectively. In this study, Aflatoxin M1(AFM1) concentration in milk was higher in (Khartoum Mean: 2.73±Std: 2.72, (Khartoum north Mean: 0.78± STD: 1.45) and Omdurman (Mean: 1.32± STD: 2.83) respectively (P<0.05).