Detection of Morganella morganii in urine Specimens by Real-Time Polymerase Chain Reaction Technique

Abstract

This study was designed to detect the presence of Morgenella morganii (M. morganii) in urine specimens by real-time polymerase chain reaction technique. The specimens were collected from patients with risk factors to develop urinary tract infection (UTI) by this organism. DNA was extracted by phenol-chloroform method. The extract was run using one set of primer for M. morganii. The result of the study showed, that M. morganii was detected in 29% of total specimens included in the study. From these 29%, males accounted for 23%, and female accounted for 6%. The ratio of hospitalized to out patients, was 25:4. The ratio of old age to presence of calculi and chronic catheterization was 8: 4: 17. The study concluded that the real-time polymerase chain reaction technique had facilitated diagnosis of UTI and it was simple, highly sensitive, specific and rapid