Effect of Enzyme Concentration and Incubation periods on Gelatin Extraction from Cattle Hides

Abstract

This study was conducted in the Nutrition laboratory, College of science and technology of Animal Production, Sudan University of Science and Technology, during April - June 2009. The aim of IIIthe study is to investigate the effects of enzyme concentration and incubation period on gelatin extraction and the possibility of gelatin extraction from cattle hides by using different concentration of pancreatic alkaline enzyme containing trypsin and pepsin (Rock enzyme 7500-L) for different incubation periods. Pieces of Limed–fleshed cattle hides weighing 5kgs were used in this study. They were cut into small pieces each 5cm2 delimed, degreased and washed. The samples were treated with different concentration of enzyme (0%, 1%, and 2%) for three different incubation periods (three, six and nine hours). The extraction continued for six hours in Water Bath at 70Co. The yield, color and pH of gelatin were measured. The study showed that the concentration of the enzyme has highly significant effect (**) at (p<0.01) on gelatin yield. The percentages of gelatin yield of the first extraction at 0%, 1% and 2% was found to be 4.33%, 6.75% and 8.6% for three hours , 4.2 %, 6.61% and 8.11% for six hours , 4.31%, 7.3% and 9.21 % for nine hours respectively . Higher yields of gelatin extraction were obtained by using 2% of enzyme. In this study the color of gelatin extracted at 0%, 1% and 2% enzyme concentration were very light creamy, light creamy and creamy, while the pH of gelatin extracted at the same concentration were in average 6.98, 6.96 and 6.98 respectively.