Isolation & Antimicrobial Sensitivity of Haemophilus influenzae in Sudanese Patients

Abstract

This study has been done on two groups of individuals for the isolation of Haemophilus influenzae from sputum. The first group of study include 50 individual patients presented with pneumonia, whilst the second group included tuberculosis patients, and those who are suspected to have tuberculosis (359) individual; The age range in both groups lie between (3-73year). Collection of the specimens was done within eight months from (April-Decimber) through 2003 from Khartoum state, and Elobied city in North Kordofan state. Nine strains of Haemophilus influenzae were isolated from the specimens. The percentage of Haemophilus influenzae isolated from the specimens was 2.2%. The percentage of ß-Lactamase producing strains of Haemophilus influenzae type b, was found to be 42.9%. The percentage of type b Haemophilus influenzae was 77.8% of the total strains of Haemophilus isolated. Furthermore, the relationship between the isolation of the organism, and the season, type of media, and age groups has been detected. The organism has been isolated in Autumn and Winter (one strain late Autumn, and eight strains in Winter), whereas no isolation took place in Summer; (GC ‫ח‬agar) was found to be the best medium for isolation compared to Chochalate agar. Children (3- 18year) and elderly(67-73year) were the most affected groups. The study proved that the organism can be preserved in (4-8) °C for about (10-15) days.