Assessment of Cellular Patterns in Aspirated Ascitic Fluid Using Three Different Cytological Stains

Abstract

This Study was carried out in Khartoum State during a period from (March to June 2005).It aimed to study cellular patterns in ascitic fluid using three cytological stains (Papanicolaou, Harris haematoxylin and eosin, and may Grun wald–Giemsa Stain). Thirty sampls were taken from patients who attended to different hospitals in Khartoum State, the sample were processed and examined microscopically. Lymphocytes were presented in 27(90%) samples, neutrophils in 7(23%), macrophage in 13(43.3%), mesothelial cell found in 18(60%) , plasma cell in 4(13.3%) cases. Eosinophils in one case (3.3%).and malignant cells in 8 samples (26.7%). The papanicolaou showed best staining (86.5%), 21 samples were evaluate as excellent , 4 very good ,2 good and 3 as acceptable . Harris haematoxylin and eosin came next (85.5%), excellent ,stain showed in 21 samples , very goods 2 samples , good 4 sample and 3 samples were acceptable . At last came, May Gun wald-Giemsa stain (80.5%).13 were evaluated as excellent, 8very good.6 good and 3 .as acceptable