Serodetection of Hepatitis A Virus among Food-handlers in Khartoum Localty

Abstract

Hepatitis A virus (HAV) infections occur mainly as a result of bad hygienic conditions. The objective of this study was to detect HAV among food handlers working in cafeterias in Khartoum Province. The study was conducted during the period from June to September 2013. Food handlers Technology working cafeterias in were Sudan included University in this of Science study. A and pretested structured questionnaire was used for collecting demographic data. Blood samples were collected from each participant, both sex's (males and females); serum was prepared from each blood sample. Enzyme linked immunosorbent assay was used to detect Anti-HAV IgM and IgG antibodies. Out of the 90 food handlers (73 males and 17 females) who took part in this study, 89 (98.8%) were positive for anti-HAV IgG, 73 (81%) form males and 16 (17.8%) from females. Moreover, only 4 (4.4%) were positive for anti-HAV IgM, 2(2.2%) form males and 2 (2.2%) from females. From the above findings, the study concluded that, there is asymptomatic individuals indicate that the HAV circulate at low but considerable levels especially among food handlers; that may be a source of outbreak. Confirmation of Hepatitis A positive food handlers should be tested polymerase chain reaction is highly recommended. Further study with large number of blood samples is required to validate the results of the present study.