Detection of Hepsin Gene in Prostate Tumors in Gezira State Using Polymerase Chain Reaction Technique

Abstract

This descriptive study was conducted in Gezira state during the period from April 2012 to July 2013.The study aimed to detect hepsin gene in prostate tumors using PCR technique. The study included 100 patients previously diagnosed as prostate tumors, divided into two groups, 50 patients diagnosed as prostate cancer as cases group and 50 patients diagnosed as benign prostate hyperplasia as control group. There were collected 2.5 ml bloods from them for DNA extraction. Their age ranged from 50 – 90 years with mean age 70.2 ± 8.9. Most of patients were in age above 65 years. The result showed that 47 patients with prostate cancer and 32 patients with benign prostate hyperplasia were above 65 years,3 patients with prostate cancer and 18 patients with benign prostate hyperplasia were less than 65 years, with significant relation between the age group and tumor behavior (P=0.000). The present study found that the expression of hepsin gene were positive in 10 patients and negative in 40 patients among case group, while in control group all cases were revealed negative expression for hepsin gene, with significant relation between the expression of hepsin gene and tumor behavior (P=0.001). This study concluded that the expression of hepsin gene is associated with prostate cancer, and most of prostate tumors patients in this study were above 65 years.