Aerobic Bacterial Contamination of Anesthetic Equipments

Abstract

This study was conducted with specific objectives of isolation and identification of bacteria associated with Anesthetic apparatus used during operations or intubations in intensive care units. Two hundred samples were collected from ten different sites of Anesthetic apparatus, Twenty specimens were taken from each sites were considered sterile and ready to use. The duration of samples collection was between (March 2001 – October 2002). Twenty specimens were taken from each site. The samples were collected from the major emergency complex theatre at Ribat University Hospital and the major theatre of Sahiroon Hospital in Khartoum state. Bacteriological examinations were performed in the microbiology laboratory, Department of Microbiology, Ribat University Hospital and Central Vet. Research Lab. Center, Soba. Both Gram-positive and Gram-negative bacteria were isolated, identified and differentiated using standard different biochemical reactions. The Gram-positive isolates were Staphylococcus spp., Micrococcus spp. and Bacillus spp.The Gram negative isolate were only Pseudomonas. The commonest bacteria that contaminate the Anesthetic apparatus were Pseudomonas spp. (60.37%), Micrococcus spp. (20.75%) and Bacillus spp. (18.38%). The antibiotic Sensitivity tests were done for each isolated strain. Pseudomonas spp. were (100%) sensitive to Amickacin, Pefloxacin and Ciprofloxacin and ( 72.7% ) sensitive to Ofloxacin and (100%) resistant to Ampicillin, Cefoperazone, Piperacillin, Ceftazidime Ceftizoxime, Augmentin, and (72.7% ) resistant to Cefotaxime and Gentamicin . Micrococcus spp. were (85.7%) sensitive to Ciprofloxacin and (71.4%) sensitive to Gentamicin, Ofloxacin and Pefloxacin, intermediately sensitive to Ceftriazone. They were found to be (85.7%) resistant to Cephalexin, (71.4%) to Ampicillin, and Lincomycin and (57.1%) resistant to Roxythromycin and Cefotaxime. Bacillus spp.were (100%) sensitive to Gentamicin and Ciprofloxacin, (85.7%) to Roxythromycin, (71.4%) to Ofloxacin and Pefloxacin and intermediately sensitive to Cephalexin and Ceftriazone .They were on the Other hand found to be (85.7%) resistant to Ampicillin and (57.1%) to Cefotaxime and Lincomycin.