Comparative Study between Kirby-Bauer, Stokes and National Committee for Clinical Laboratory Standanderdization, Reading Methods of Antimicrobial Susceptibility Testing

Abstract

The main objectives of this study were to compare the results obtained by different methods carried for detecting the antimicrobial sensitivity testing (NCCLS table diameter method, Stokes and Kirby-Bauer comparative methods) and to compare the results when using two standard media (Muller-Hinton and Diagnostic sensitivity agar). Fifty organisms were isolated and identified to reach these objectives. They were from two different sites, urine, and wounds. The isolates were: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, Staphylococcus aureus and Enterococcus faecalis. Antimicrobial sensitivity testing was carried out for each organism by the three methods on the two media. The results were recorded and analyzed to detect the differences. According to the statistical analysis, no differences were found to be between the comparative methods Stokes and Kirby-Bauer, while a significant difference was reported between them and NCCLS method. When ‫‪comparing the two media, no differences were noted, and the‬‬ ‫.‪results were found to be similar‬‬