Isolation of ِAnamorph of Pseudallescheria boydii from Different Environmental Sources in Khartoum State - Sudan

Abstract

This study was conducted in Sudan University of Science and Technology, Khartoum, Sudan, in collaboration with Centraalbureau voor Schimmelcultures (CBS), Fungal Biodiversity Center, Utrecht, The Netherlands, during the period from June to August 2005. The study aimed of isolating Pseudallescheria boydii from soil and polluted water specimens collected from Sudan. Twenty two dry soil and fourteen polluted water specimens were collected from Khartoum State, Khartoum, Sudan. Diluted specimens suspensions were inoculated directly onto SeScel medium with and without Benomyl, and incubated at 36oC for one week. Colonies resembling P. boydii were purified on Malt extract agar and identified. No teleomorph or anamorph were isolated form soil specimen (n=22), while 5 Scedosporium apiospermum and Graphium eumorphum isolates were isolated from polluted water (n=14). The identification of the isolates was confirmed by PCR and DNA sequencing. This study documented the occurrence of P. boydii in the Sudanese environment, which should increase the awareness of clinicians and microbiologists to negative impact of this relatively resistant fungus.