Assessment of the Significance of using Microwave oven in Melanin Staining Techniques as Time Reducing Factor in Masson-Fontana Method

Abstract

This a retrospective study was carried out the research laboratory, department of Histopathology and Cytology in Sudan University of science and technology, Khartoum Sudan. The objective of this study was to evaluate the effects of Microwave oven in Melanin pigment staining procedures and to compare between the classical Masson-Fontana staining technique at room temperature and Microwave technique with Masson-Fontana stain. 50 skin tissue samples were taken for processing. After processing, two histochemical staining techniques were carried out ;classical Masson-Fontana method at room temperature and Microwave oven Masson-Fontana staining method at temperature 20c. Both methods were compared. The results of this study, indicated that the use of microwave oven in staining of melanin pigment reduced the time from overnight to only 2minutes with very good staining quality as compared of classical Masson-Fontana method. Using Harris haemaoxylin and Eosin stain for histological changes examination and diagnosis conformation. In conclusion, the use of microwave oven is a vital tool for staining of melanin with Masson-Fontana method. Further research and studies to improve the application of microwave oven in the staining of melanin pigment with Masson-Fontana stain are recommended.