Detection of Salmonella typhimurium in Stool Specimens by Real time Polymerase Chain Reaction

Abstract

This study was carried out in the period from May 2007- March 2008 to detect the presence of Salmonella typhimurium in stool of patients who were suffering from acute gastrointestinal tract infection. Forty seven stool specimens were collected from patients who were attended to Bashair Hospital, Omdrman Teaching Hospital and Haj Alsafi Hospital during the study period. Bacterial DNA was extracted from each stool specimen.The DNA was runned using real time PCR technique to detect S .typhimurium The result revealed that only two specimens were positive. The study concluded that the real time PCR technique facilitates detection of S .typhimurium without bacteriological culture.