Measurement of Some Hematological Parameters and Hemoglobin Electrophoresis among Patients with Sickle Cell Anemia Attending Um Rawaba Teaching Hospital

Abstract

This is a cross-sectional descriptive analytical study conducted at Um Rawaba city from May to August 2013.Study aimed to measure some hematological parameters and hemoglobin electrophoresis of patients with sickle cell anemia attending Um Rawaba Teaching Hospital. Sixty four blood samples were collected,; fifty for sicklers 43 SS and 7 AS fourteen for apparently healthy individuals as control .Aquestionnaire was designed to collect information about the study, 3ml of venous blood was collected in EDTA anticoagulant container and automated hematological analyzer –Sysmex 21 - was used to investigate the hematological parameters. Blood sample was washed three times with normal saline for preparing it for hemoglobin electrophoresis test . The results conducted that the mean for Hb (6.4 g/dl), RBCS (2.82 × 1012 /L) , and PCV (23.41%) of patients with sickle cell anemia are decreased significantly compared to control group, Hb (12.24 g/dl), RBCS (4.72 × 1012 /L) , and PCV (41.06%) (P. value 0.005), WBC counts (17.36 × 109 /L) of sickle cell anemic patients are increased significantly compared to control group WBC counts (5.54 × 109 /L). The MCV (84.55 FL) and MCH (23.41 pg) of sickle cell anemic patients are decreased insignificantly compared to control group MCV (85.59 FL) MCH (25.44 pg ).The MCHC (27.53 g/dl) of patients are decreased significantly compared to control group MCHC (30.32 g/dl). The platelets (ptt 357.56× 103 /mm3 ) of sickle cell anemic patients increased are insignificantly compared to control group (ptt 307.21 × 103 / mm3) . Some finding related to age, and sex and tribe were obtained according to social data .inter-marriage played an important role in spreading of the sickle cell disease and the sickle cell disease is more common in Gwama ,Baggara ,Falata,Habania .Rezygat and Bedaria tribes.